A highly selective fluorescent probe for thiol bioimaging

Org Lett. 2008 Jan 3;10(1):37-40. doi: 10.1021/ol702539v. Epub 2007 Dec 7.

Abstract

A new fluorescent turn-on probe (3) for the selective sensing and bioimaging of thiols is reported. In aqueous buffer solutions at physiological pH, thiols cleave the 2,4-dinitrobenzenesulfonyl group to release the red-emissive donor-acceptor fluorophore (4). The probe displays excellent immunity to interference from nitrogen and oxygen nucleophiles and the imaging of thiols in living cells is demonstrated.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Colorimetry / methods
  • Diagnostic Imaging / methods*
  • Fluorescent Dyes*
  • Molecular Structure
  • Nitrobenzenes / chemistry*
  • Nitrogen / chemistry
  • Oxygen / chemistry
  • Sulfhydryl Compounds / analysis*

Substances

  • Fluorescent Dyes
  • Nitrobenzenes
  • Sulfhydryl Compounds
  • Nitrogen
  • Oxygen